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Torin 1 mTOR remmer
Cat.nr.S2827
Chemische structuur
Molecuulgewicht: 607.62
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Kwaliteitscontrole
Batch:
Zuiverheid:
99.07%
99.07
| Gerelateerde doelwitten | PI3K Akt GSK-3 ATM/ATR DNA-PK AMPK PDPK1 PTEN PP2A PDK |
|---|---|
| Overig mTOR Inhibitoren | Torin 2 AZD8055 Ridaforolimus (Deforolimus, MK-8669) Sapanisertib (MLN0128, INK-128) Torkinib (PP242) MHY1485 Vistusertib (AZD2014) KU-0063794 OSI-027 WYE-354 |
Celkweek, behandeling & werkzame concentratie
| Cellijnen | Assaytype | Concentratie | Incubatietijd | Formulering | Activiteitsbeschrijving | PMID |
|---|---|---|---|---|---|---|
| ETK-1 | Growth Inhibition Assay | IC50=0.000306 μM | SANGER | |||
| OCUB-M | Growth Inhibition Assay | IC50=0.00194 μM | SANGER | |||
| DOHH-2 | Growth Inhibition Assay | IC50=0.00225 μM | SANGER | |||
| NOS-1 | Growth Inhibition Assay | IC50=0.00242 μM | SANGER | |||
| L-363 | Growth Inhibition Assay | IC50=0.00284 μM | SANGER | |||
| RL95-2 | Growth Inhibition Assay | IC50=0.0032 μM | SANGER | |||
| BL-70 | Growth Inhibition Assay | IC50=0.00337 μM | SANGER | |||
| TGBC1TKB | Growth Inhibition Assay | IC50=0.00379 μM | SANGER | |||
| 697 | Growth Inhibition Assay | IC50=0.00634 μM | SANGER | |||
| RPMI-8226 | Growth Inhibition Assay | IC50=0.00651 μM | SANGER | |||
| BL-41 | Growth Inhibition Assay | IC50=0.00696 μM | SANGER | |||
| MRK-nu-1 | Growth Inhibition Assay | IC50=0.00707 μM | SANGER | |||
| EW-1 | Growth Inhibition Assay | IC50=0.00749 μM | SANGER | |||
| EVSA-T | Growth Inhibition Assay | IC50=0.00892 μM | SANGER | |||
| HUTU-80 | Growth Inhibition Assay | IC50=0.00929 μM | SANGER | |||
| VA-ES-BJ | Growth Inhibition Assay | IC50=0.00984 μM | SANGER | |||
| GCIY | Growth Inhibition Assay | IC50=0.01007 μM | SANGER | |||
| ES6 | Growth Inhibition Assay | IC50=0.01083 μM | SANGER | |||
| NALM-6 | Growth Inhibition Assay | IC50=0.01102 μM | SANGER | |||
| LB996-RCC | Growth Inhibition Assay | IC50=0.01137 μM | SANGER | |||
| EW-16 | Growth Inhibition Assay | IC50=0.01192 μM | SANGER | |||
| KALS-1 | Growth Inhibition Assay | IC50=0.01198 μM | SANGER | |||
| OPM-2 | Growth Inhibition Assay | IC50=0.0129 μM | SANGER | |||
| KU812 | Growth Inhibition Assay | IC50=0.01296 μM | SANGER | |||
| A4-Fuk | Growth Inhibition Assay | IC50=0.01319 μM | SANGER | |||
| KGN | Growth Inhibition Assay | IC50=0.01359 μM | SANGER | |||
| KS-1 | Growth Inhibition Assay | IC50=0.01392 μM | SANGER | |||
| no-11 | Growth Inhibition Assay | IC50=0.01395 μM | SANGER | |||
| PSN1 | Growth Inhibition Assay | IC50=0.01409 μM | SANGER | |||
| SK-LMS-1 | Growth Inhibition Assay | IC50=0.01413 μM | SANGER | |||
| LS-411N | Growth Inhibition Assay | IC50=0.01439 μM | SANGER | |||
| HT-144 | Growth Inhibition Assay | IC50=0.01451 μM | SANGER | |||
| ACN | Growth Inhibition Assay | IC50=0.015 μM | SANGER | |||
| RKO | Growth Inhibition Assay | IC50=0.01533 μM | SANGER | |||
| CMK | Growth Inhibition Assay | IC50=0.01543 μM | SANGER | |||
| GB-1 | Growth Inhibition Assay | IC50=0.01553 μM | SANGER | |||
| KE-37 | Growth Inhibition Assay | IC50=0.01573 μM | SANGER | |||
| no-10 | Growth Inhibition Assay | IC50=0.01584 μM | SANGER | |||
| MOLT-16 | Growth Inhibition Assay | IC50=0.01622 μM | SANGER | |||
| HEL | Growth Inhibition Assay | IC50=0.0164 μM | SANGER | |||
| NCI-H1355 | Growth Inhibition Assay | IC50=0.01646 μM | SANGER | |||
| IST-MEL1 | Growth Inhibition Assay | IC50=0.01774 μM | SANGER | |||
| MHH-PREB-1 | Growth Inhibition Assay | IC50=0.01818 μM | SANGER | |||
| ES8 | Growth Inhibition Assay | IC50=0.01844 μM | SANGER | |||
| LC-2-ad | Growth Inhibition Assay | IC50=0.02137 μM | SANGER | |||
| LAMA-84 | Growth Inhibition Assay | IC50=0.02167 μM | SANGER | |||
| BV-173 | Growth Inhibition Assay | IC50=0.02203 μM | SANGER | |||
| SW872 | Growth Inhibition Assay | IC50=0.02247 μM | SANGER | |||
| SK-UT-1 | Growth Inhibition Assay | IC50=0.02261 μM | SANGER | |||
| OS-RC-2 | Growth Inhibition Assay | IC50=0.02323 μM | SANGER | |||
| NCI-H747 | Growth Inhibition Assay | IC50=0.0233 μM | SANGER | |||
| MZ2-MEL | Growth Inhibition Assay | IC50=0.02337 μM | SANGER | |||
| TE-11 | Growth Inhibition Assay | IC50=0.02372 μM | SANGER | |||
| KURAMOCHI | Growth Inhibition Assay | IC50=0.02414 μM | SANGER | |||
| HD-MY-Z | Growth Inhibition Assay | IC50=0.02426 μM | SANGER | |||
| GI-1 | Growth Inhibition Assay | IC50=0.02469 μM | SANGER | |||
| LB1047-RCC | Growth Inhibition Assay | IC50=0.02488 μM | SANGER | |||
| TE-12 | Growth Inhibition Assay | IC50=0.0255 μM | SANGER | |||
| MZ7-mel | Growth Inhibition Assay | IC50=0.02609 μM | SANGER | |||
| MMAC-SF | Growth Inhibition Assay | IC50=0.02633 μM | SANGER | |||
| A101D | Growth Inhibition Assay | IC50=0.0265 μM | SANGER | |||
| GR-ST | Growth Inhibition Assay | IC50=0.02655 μM | SANGER | |||
| MC116 | Growth Inhibition Assay | IC50=0.0268 μM | SANGER | |||
| Ramos-2G6-4C10 | Growth Inhibition Assay | IC50=0.0275 μM | SANGER | |||
| ATN-1 | Growth Inhibition Assay | IC50=0.02789 μM | SANGER | |||
| MFH-ino | Growth Inhibition Assay | IC50=0.02812 μM | SANGER | |||
| DEL | Growth Inhibition Assay | IC50=0.02815 μM | SANGER | |||
| OCI-AML2 | Growth Inhibition Assay | IC50=0.02871 μM | SANGER | |||
| LXF-289 | Growth Inhibition Assay | IC50=0.02872 μM | SANGER | |||
| IA-LM | Growth Inhibition Assay | IC50=0.02917 μM | SANGER | |||
| EoL-1-cell | Growth Inhibition Assay | IC50=0.02996 μM | SANGER | |||
| OVCAR-4 | Growth Inhibition Assay | IC50=0.03094 μM | SANGER | |||
| ES1 | Growth Inhibition Assay | IC50=0.03097 μM | SANGER | |||
| CESS | Growth Inhibition Assay | IC50=0.03099 μM | SANGER | |||
| GAK | Growth Inhibition Assay | IC50=0.03186 μM | SANGER | |||
| NTERA-S-cl-D1 | Growth Inhibition Assay | IC50=0.03226 μM | SANGER | |||
| TE-10 | Growth Inhibition Assay | IC50=0.03311 μM | SANGER | |||
| CCRF-CEM | Growth Inhibition Assay | IC50=0.03371 μM | SANGER | |||
| NMC-G1 | Growth Inhibition Assay | IC50=0.03448 μM | SANGER | |||
| HOP-62 | Growth Inhibition Assay | IC50=0.03556 μM | SANGER | |||
| SH-4 | Growth Inhibition Assay | IC50=0.03582 μM | SANGER | |||
| NCI-H1648 | Growth Inhibition Assay | IC50=0.03622 μM | SANGER | |||
| EB-3 | Growth Inhibition Assay | IC50=0.03643 μM | SANGER | |||
| BB65-RCC | Growth Inhibition Assay | IC50=0.03691 μM | SANGER | |||
| BE-13 | Growth Inhibition Assay | IC50=0.03759 μM | SANGER | |||
| CP67-MEL | Growth Inhibition Assay | IC50=0.03759 μM | SANGER | |||
| SW954 | Growth Inhibition Assay | IC50=0.0379 μM | SANGER | |||
| TE-5 | Growth Inhibition Assay | IC50=0.03854 μM | SANGER | |||
| HCE-4 | Growth Inhibition Assay | IC50=0.03855 μM | SANGER | |||
| LC-1F | Growth Inhibition Assay | IC50=0.03989 μM | SANGER | |||
| TK10 | Growth Inhibition Assay | IC50=0.04019 μM | SANGER | |||
| SJSA-1 | Growth Inhibition Assay | IC50=0.04188 μM | SANGER | |||
| Daudi | Growth Inhibition Assay | IC50=0.04225 μM | SANGER | |||
| SW982 | Growth Inhibition Assay | IC50=0.04246 μM | SANGER | |||
| SF126 | Growth Inhibition Assay | IC50=0.04361 μM | SANGER | |||
| TE-15 | Growth Inhibition Assay | IC50=0.04435 μM | SANGER | |||
| COLO-684 | Growth Inhibition Assay | IC50=0.04561 μM | SANGER | |||
| 8-MG-BA | Growth Inhibition Assay | IC50=0.04629 μM | SANGER | |||
| NB13 | Growth Inhibition Assay | IC50=0.04776 μM | SANGER | |||
| HCC1599 | Growth Inhibition Assay | IC50=0.04879 μM | SANGER | |||
| SK-PN-DW | Growth Inhibition Assay | IC50=0.04949 μM | SANGER | |||
| ALL-PO | Growth Inhibition Assay | IC50=0.04956 μM | SANGER | |||
| NB6 | Growth Inhibition Assay | IC50=0.05271 μM | SANGER | |||
| IST-SL2 | Growth Inhibition Assay | IC50=0.05362 μM | SANGER | |||
| BB30-HNC | Growth Inhibition Assay | IC50=0.0573 μM | SANGER | |||
| SW962 | Growth Inhibition Assay | IC50=0.05763 μM | SANGER | |||
| TGBC24TKB | Growth Inhibition Assay | IC50=0.05799 μM | SANGER | |||
| KINGS-1 | Growth Inhibition Assay | IC50=0.05803 μM | SANGER | |||
| HC-1 | Growth Inhibition Assay | IC50=0.05827 μM | SANGER | |||
| RS4-11 | Growth Inhibition Assay | IC50=0.05938 μM | SANGER | |||
| LS-123 | Growth Inhibition Assay | IC50=0.05942 μM | SANGER | |||
| A3-KAW | Growth Inhibition Assay | IC50=0.06225 μM | SANGER | |||
| SNB75 | Growth Inhibition Assay | IC50=0.06341 μM | SANGER | |||
| SR | Growth Inhibition Assay | IC50=0.06374 μM | SANGER | |||
| LOUCY | Growth Inhibition Assay | IC50=0.06394 μM | SANGER | |||
| K5 | Growth Inhibition Assay | IC50=0.06403 μM | SANGER | |||
| Becker | Growth Inhibition Assay | IC50=0.0642 μM | SANGER | |||
| CTV-1 | Growth Inhibition Assay | IC50=0.06548 μM | SANGER | |||
| KLE | Growth Inhibition Assay | IC50=0.06584 μM | SANGER | |||
| MZ1-PC | Growth Inhibition Assay | IC50=0.06598 μM | SANGER | |||
| MOLT-4 | Growth Inhibition Assay | IC50=0.06605 μM | SANGER | |||
| MLMA | Growth Inhibition Assay | IC50=0.0678 μM | SANGER | |||
| A388 | Growth Inhibition Assay | IC50=0.06893 μM | SANGER | |||
| MV-4-11 | Growth Inhibition Assay | IC50=0.06995 μM | SANGER | |||
| DSH1 | Growth Inhibition Assay | IC50=0.07173 μM | SANGER | |||
| MONO-MAC-6 | Growth Inhibition Assay | IC50=0.07212 μM | SANGER | |||
| ARH-77 | Growth Inhibition Assay | IC50=0.07278 μM | SANGER | |||
| LB771-HNC | Growth Inhibition Assay | IC50=0.07549 μM | SANGER | |||
| PF-382 | Growth Inhibition Assay | IC50=0.07569 μM | SANGER | |||
| KM12 | Growth Inhibition Assay | IC50=0.0764 μM | SANGER | |||
| HAL-01 | Growth Inhibition Assay | IC50=0.07658 μM | SANGER | |||
| RPMI-8402 | Growth Inhibition Assay | IC50=0.07789 μM | SANGER | |||
| OMC-1 | Growth Inhibition Assay | IC50=0.07804 μM | SANGER | |||
| HT | Growth Inhibition Assay | IC50=0.08 μM | SANGER | |||
| QIMR-WIL | Growth Inhibition Assay | IC50=0.08055 μM | SANGER | |||
| KNS-42 | Growth Inhibition Assay | IC50=0.08203 μM | SANGER | |||
| BC-3 | Growth Inhibition Assay | IC50=0.08204 μM | SANGER | |||
| ES4 | Growth Inhibition Assay | IC50=0.08296 μM | SANGER | |||
| NCI-H23 | Growth Inhibition Assay | IC50=0.0844 μM | SANGER | |||
| LB2518-MEL | Growth Inhibition Assay | IC50=0.08696 μM | SANGER | |||
| LC4-1 | Growth Inhibition Assay | IC50=0.08738 μM | SANGER | |||
| SIG-M5 | Growth Inhibition Assay | IC50=0.08904 μM | SANGER | |||
| LP-1 | Growth Inhibition Assay | IC50=0.08938 μM | SANGER | |||
| TE-8 | Growth Inhibition Assay | IC50=0.09016 μM | SANGER | |||
| K-562 | Growth Inhibition Assay | IC50=0.0912 μM | SANGER | |||
| J-RT3-T3-5 | Growth Inhibition Assay | IC50=0.09153 μM | SANGER | |||
| DU-4475 | Growth Inhibition Assay | IC50=0.09165 μM | SANGER | |||
| RXF393 | Growth Inhibition Assay | IC50=0.09462 μM | SANGER | |||
| NB5 | Growth Inhibition Assay | IC50=0.09625 μM | SANGER | |||
| A704 | Growth Inhibition Assay | IC50=0.09741 μM | SANGER | |||
| SF268 | Growth Inhibition Assay | IC50=0.09825 μM | SANGER | |||
| UACC-257 | Growth Inhibition Assay | IC50=0.09833 μM | SANGER | |||
| L-428 | Growth Inhibition Assay | IC50=0.1032 μM | SANGER | |||
| ST486 | Growth Inhibition Assay | IC50=0.10606 μM | SANGER | |||
| SK-MM-2 | Growth Inhibition Assay | IC50=0.10709 μM | SANGER | |||
| COR-L279 | Growth Inhibition Assay | IC50=0.10832 μM | SANGER | |||
| JiyoyeP-2003 | Growth Inhibition Assay | IC50=0.10843 μM | SANGER | |||
| GT3TKB | Growth Inhibition Assay | IC50=0.10918 μM | SANGER | |||
| HCE-T | Growth Inhibition Assay | IC50=0.1094 μM | SANGER | |||
| SBC-1 | Growth Inhibition Assay | IC50=0.11119 μM | SANGER | |||
| KNS-81-FD | Growth Inhibition Assay | IC50=0.11273 μM | SANGER | |||
| NCI-H2126 | Growth Inhibition Assay | IC50=0.11394 μM | SANGER | |||
| EM-2 | Growth Inhibition Assay | IC50=0.11529 μM | SANGER | |||
| JAR | Growth Inhibition Assay | IC50=0.11598 μM | SANGER | |||
| NB17 | Growth Inhibition Assay | IC50=0.11652 μM | SANGER | |||
| LB647-SCLC | Growth Inhibition Assay | IC50=0.11705 μM | SANGER | |||
| CAL-148 | Growth Inhibition Assay | IC50=0.118 μM | SANGER | |||
| K052 | Growth Inhibition Assay | IC50=0.11851 μM | SANGER | |||
| KARPAS-299 | Growth Inhibition Assay | IC50=0.12233 μM | SANGER | |||
| KARPAS-422 | Growth Inhibition Assay | IC50=0.12432 μM | SANGER | |||
| LOXIMVI | Growth Inhibition Assay | IC50=0.12515 μM | SANGER | |||
| SCC-3 | Growth Inhibition Assay | IC50=0.12851 μM | SANGER | |||
| LU-139 | Growth Inhibition Assay | IC50=0.12975 μM | SANGER | |||
| COLO-320-HSR | Growth Inhibition Assay | IC50=0.13086 μM | SANGER | |||
| ONS-76 | Growth Inhibition Assay | IC50=0.1313 μM | SANGER | |||
| U-266 | Growth Inhibition Assay | IC50=0.13245 μM | SANGER | |||
| HH | Growth Inhibition Assay | IC50=0.13535 μM | SANGER | |||
| BB49-HNC | Growth Inhibition Assay | IC50=0.13587 μM | SANGER | |||
| NCI-SNU-5 | Growth Inhibition Assay | IC50=0.13596 μM | SANGER | |||
| C2BBe1 | Growth Inhibition Assay | IC50=0.13746 μM | SANGER | |||
| ML-2 | Growth Inhibition Assay | IC50=0.13953 μM | SANGER | |||
| SCH | Growth Inhibition Assay | IC50=0.13974 μM | SANGER | |||
| NCI-H82 | Growth Inhibition Assay | IC50=0.14104 μM | SANGER | |||
| MDA-MB-134-VI | Growth Inhibition Assay | IC50=0.14252 μM | SANGER | |||
| SK-N-DZ | Growth Inhibition Assay | IC50=0.14719 μM | SANGER | |||
| HL-60 | Growth Inhibition Assay | IC50=0.1492 μM | SANGER | |||
| LB373-MEL-D | Growth Inhibition Assay | IC50=0.15539 μM | SANGER | |||
| LB831-BLC | Growth Inhibition Assay | IC50=0.16076 μM | SANGER | |||
| SK-NEP-1 | Growth Inhibition Assay | IC50=0.16101 μM | SANGER | |||
| IMR-5 | Growth Inhibition Assay | IC50=0.16483 μM | SANGER | |||
| Calu-6 | Growth Inhibition Assay | IC50=0.16537 μM | SANGER | |||
| NCI-H1882 | Growth Inhibition Assay | IC50=0.16728 μM | SANGER | |||
| NCI-H446 | Growth Inhibition Assay | IC50=0.16891 μM | SANGER | |||
| GI-ME-N | Growth Inhibition Assay | IC50=0.1802 μM | SANGER | |||
| MPP-89 | Growth Inhibition Assay | IC50=0.18121 μM | SANGER | |||
| CGTH-W-1 | Growth Inhibition Assay | IC50=0.18436 μM | SANGER | |||
| MEG-01 | Growth Inhibition Assay | IC50=0.18628 μM | SANGER | |||
| NCI-H748 | Growth Inhibition Assay | IC50=0.18911 μM | SANGER | |||
| COLO-829 | Growth Inhibition Assay | IC50=0.20673 μM | SANGER | |||
| GOTO | Growth Inhibition Assay | IC50=0.2073 μM | SANGER | |||
| EW-3 | Growth Inhibition Assay | IC50=0.21718 μM | SANGER | |||
| KY821 | Growth Inhibition Assay | IC50=0.22045 μM | SANGER | |||
| CTB-1 | Growth Inhibition Assay | IC50=0.22228 μM | SANGER | |||
| TALL-1 | Growth Inhibition Assay | IC50=0.22921 μM | SANGER | |||
| NCI-H209 | Growth Inhibition Assay | IC50=0.22962 μM | SANGER | |||
| IST-MES1 | Growth Inhibition Assay | IC50=0.2351 μM | SANGER | |||
| DMS-114 | Growth Inhibition Assay | IC50=0.23993 μM | SANGER | |||
| SNU-C2B | Growth Inhibition Assay | IC50=0.24316 μM | SANGER | |||
| DG-75 | Growth Inhibition Assay | IC50=0.26195 μM | SANGER | |||
| D-336MG | Growth Inhibition Assay | IC50=0.26557 μM | SANGER | |||
| KMOE-2 | Growth Inhibition Assay | IC50=0.27538 μM | SANGER | |||
| NB1 | Growth Inhibition Assay | IC50=0.27926 μM | SANGER | |||
| RH-1 | Growth Inhibition Assay | IC50=0.27989 μM | SANGER | |||
| TE-1 | Growth Inhibition Assay | IC50=0.28479 μM | SANGER | |||
| RPMI-6666 | Growth Inhibition Assay | IC50=0.2884 μM | SANGER | |||
| TE-9 | Growth Inhibition Assay | IC50=0.28985 μM | SANGER | |||
| BT-474 | Growth Inhibition Assay | IC50=0.29334 μM | SANGER | |||
| COLO-668 | Growth Inhibition Assay | IC50=0.29429 μM | SANGER | |||
| NCI-H1581 | Growth Inhibition Assay | IC50=0.29689 μM | SANGER | |||
| MN-60 | Growth Inhibition Assay | IC50=0.29905 μM | SANGER | |||
| L-540 | Growth Inhibition Assay | IC50=0.30081 μM | SANGER | |||
| MOLT-13 | Growth Inhibition Assay | IC50=0.30183 μM | SANGER | |||
| NCI-SNU-16 | Growth Inhibition Assay | IC50=0.30205 μM | SANGER | |||
| NCI-H64 | Growth Inhibition Assay | IC50=0.30331 μM | SANGER | |||
| CP66-MEL | Growth Inhibition Assay | IC50=0.31415 μM | SANGER | |||
| NB69 | Growth Inhibition Assay | IC50=0.32188 μM | SANGER | |||
| NCI-H2196 | Growth Inhibition Assay | IC50=0.33036 μM | SANGER | |||
| MHH-CALL-2 | Growth Inhibition Assay | IC50=0.33468 μM | SANGER | |||
| HCC2157 | Growth Inhibition Assay | IC50=0.33954 μM | SANGER | |||
| LS-1034 | Growth Inhibition Assay | IC50=0.36388 μM | SANGER | |||
| BOKU | Growth Inhibition Assay | IC50=0.37199 μM | SANGER | |||
| GDM-1 | Growth Inhibition Assay | IC50=0.37234 μM | SANGER | |||
| TGW | Growth Inhibition Assay | IC50=0.37453 μM | SANGER | |||
| SNU-C1 | Growth Inhibition Assay | IC50=0.38215 μM | SANGER | |||
| LS-513 | Growth Inhibition Assay | IC50=0.39071 μM | SANGER | |||
| NOMO-1 | Growth Inhibition Assay | IC50=0.39971 μM | SANGER | |||
| MS-1 | Growth Inhibition Assay | IC50=0.40177 μM | SANGER | |||
| EKVX | Growth Inhibition Assay | IC50=0.44461 μM | SANGER | |||
| SK-MEL-2 | Growth Inhibition Assay | IC50=0.464 μM | SANGER | |||
| HCC1187 | Growth Inhibition Assay | IC50=0.46768 μM | SANGER | |||
| HCC2218 | Growth Inhibition Assay | IC50=0.47227 μM | SANGER | |||
| NCI-H187 | Growth Inhibition Assay | IC50=0.4784 μM | SANGER | |||
| DB | Growth Inhibition Assay | IC50=0.50055 μM | SANGER | |||
| NCI-H69 | Growth Inhibition Assay | IC50=0.50247 μM | SANGER | |||
| NCI-H1694 | Growth Inhibition Assay | IC50=0.50697 μM | SANGER | |||
| CA46 | Growth Inhibition Assay | IC50=0.50917 μM | SANGER | |||
| EHEB | Growth Inhibition Assay | IC50=0.51193 μM | SANGER | |||
| NCCIT | Growth Inhibition Assay | IC50=0.54321 μM | SANGER | |||
| NCI-H720 | Growth Inhibition Assay | IC50=0.54962 μM | SANGER | |||
| LU-134-A | Growth Inhibition Assay | IC50=0.55296 μM | SANGER | |||
| MC-CAR | Growth Inhibition Assay | IC50=0.55431 μM | SANGER | |||
| NEC8 | Growth Inhibition Assay | IC50=0.56172 μM | SANGER | |||
| NCI-H524 | Growth Inhibition Assay | IC50=0.58748 μM | SANGER | |||
| NCI-H1963 | Growth Inhibition Assay | IC50=0.6258 μM | SANGER | |||
| SU-DHL-1 | Growth Inhibition Assay | IC50=0.64526 μM | SANGER | |||
| NCI-H1522 | Growth Inhibition Assay | IC50=0.64883 μM | SANGER | |||
| NCI-H1155 | Growth Inhibition Assay | IC50=0.6535 μM | SANGER | |||
| D-263MG | Growth Inhibition Assay | IC50=0.66101 μM | SANGER | |||
| TE-6 | Growth Inhibition Assay | IC50=0.68104 μM | SANGER | |||
| NCI-H1417 | Growth Inhibition Assay | IC50=0.69711 μM | SANGER | |||
| NCI-H510A | Growth Inhibition Assay | IC50=0.77236 μM | SANGER | |||
| KARPAS-45 | Growth Inhibition Assay | IC50=0.78235 μM | SANGER | |||
| IST-SL1 | Growth Inhibition Assay | IC50=0.85825 μM | SANGER | |||
| COLO-824 | Growth Inhibition Assay | IC50=0.87835 μM | SANGER | |||
| NCI-H526 | Growth Inhibition Assay | IC50=0.90958 μM | SANGER | |||
| NCI-H719 | Growth Inhibition Assay | IC50=0.96657 μM | SANGER | |||
| SF539 | Growth Inhibition Assay | IC50=0.98396 μM | SANGER | |||
| NKM-1 | Growth Inhibition Assay | IC50=0.98663 μM | SANGER | |||
| A253 | Growth Inhibition Assay | IC50=0.98885 μM | SANGER | |||
| TE-441-T | Growth Inhibition Assay | IC50=1.0435 μM | SANGER | |||
| COR-L88 | Growth Inhibition Assay | IC50=1.11354 μM | SANGER | |||
| CPC-N | Growth Inhibition Assay | IC50=1.15888 μM | SANGER | |||
| NCI-H2107 | Growth Inhibition Assay | IC50=1.17261 μM | SANGER | |||
| ECC4 | Growth Inhibition Assay | IC50=1.18693 μM | SANGER | |||
| NCI-H2141 | Growth Inhibition Assay | IC50=1.19299 μM | SANGER | |||
| NCI-H1770 | Growth Inhibition Assay | IC50=1.32928 μM | SANGER | |||
| NCI-SNU-1 | Growth Inhibition Assay | IC50=1.3304 μM | SANGER | |||
| LU-165 | Growth Inhibition Assay | IC50=1.45467 μM | SANGER | |||
| Raji | Growth Inhibition Assay | IC50=1.47259 μM | SANGER | |||
| EW-24 | Growth Inhibition Assay | IC50=1.54619 μM | SANGER | |||
| KP-N-RT-BM-1 | Growth Inhibition Assay | IC50=1.56551 μM | SANGER | |||
| SK-MEL-1 | Growth Inhibition Assay | IC50=1.64877 μM | SANGER | |||
| SCLC-21H | Growth Inhibition Assay | IC50=1.66651 μM | SANGER | |||
| SIMA | Growth Inhibition Assay | IC50=1.68233 μM | SANGER | |||
| NCI-H1304 | Growth Inhibition Assay | IC50=1.70765 μM | SANGER | |||
| REH | Growth Inhibition Assay | IC50=1.71078 μM | SANGER | |||
| KP-N-YS | Growth Inhibition Assay | IC50=1.93551 μM | SANGER | |||
| DMS-153 | Growth Inhibition Assay | IC50=1.9759 μM | SANGER | |||
| NB10 | Growth Inhibition Assay | IC50=2.20878 μM | SANGER | |||
| NCI-H345 | Growth Inhibition Assay | IC50=2.61905 μM | SANGER | |||
| SK-N-FI | Growth Inhibition Assay | IC50=2.75381 μM | SANGER | |||
| NCI-H1092 | Growth Inhibition Assay | IC50=2.95394 μM | SANGER | |||
| KM-H2 | Growth Inhibition Assay | IC50=3.65396 μM | SANGER | |||
| KG-1 | Growth Inhibition Assay | IC50=3.71688 μM | SANGER | |||
| D-283MED | Growth Inhibition Assay | IC50=3.7587 μM | SANGER | |||
| LU-65 | Growth Inhibition Assay | IC50=4.11485 μM | SANGER | |||
| MSTO-211H | Growth Inhibition Assay | IC50=4.18898 μM | SANGER | |||
| NCI-H2081 | Growth Inhibition Assay | IC50=4.29174 μM | SANGER | |||
| D-247MG | Growth Inhibition Assay | IC50=4.43191 μM | SANGER | |||
| P31-FUJ | Growth Inhibition Assay | IC50=4.45019 μM | SANGER | |||
| DMS-79 | Growth Inhibition Assay | IC50=4.93183 μM | SANGER | |||
| KASUMI-1 | Growth Inhibition Assay | IC50=4.97509 μM | SANGER | |||
| JVM-3 | Growth Inhibition Assay | IC50=5.25014 μM | SANGER | |||
| LAN-6 | Growth Inhibition Assay | IC50=5.51736 μM | SANGER | |||
| DJM-1 | Growth Inhibition Assay | IC50=5.56029 μM | SANGER | |||
| JVM-2 | Growth Inhibition Assay | IC50=5.67092 μM | SANGER | |||
| U-87-MG | Growth Inhibition Assay | IC50=5.89964 μM | SANGER | |||
| SHP-77 | Growth Inhibition Assay | IC50=5.94928 μM | SANGER | |||
| RPMI-8866 | Growth Inhibition Assay | IC50=6.27013 μM | SANGER | |||
| ES7 | Growth Inhibition Assay | IC50=6.39924 μM | SANGER | |||
| WSU-NHL | Growth Inhibition Assay | IC50=6.51609 μM | SANGER | |||
| CAS-1 | Growth Inhibition Assay | IC50=7.09749 μM | SANGER | |||
| MHH-NB-11 | Growth Inhibition Assay | IC50=8.23152 μM | SANGER | |||
| ES5 | Growth Inhibition Assay | IC50=9.52286 μM | SANGER | |||
| HDLM-2 | Growth Inhibition Assay | IC50=9.95902 μM | SANGER | |||
| NCI-H2171 | Growth Inhibition Assay | IC50=10.5927 μM | SANGER | |||
| LNCaP-Clone-FGC | Growth Inhibition Assay | IC50=11.3718 μM | SANGER | |||
| NCI-H1395 | Growth Inhibition Assay | IC50=12.2699 μM | SANGER | |||
| NCI-H1436 | Growth Inhibition Assay | IC50=13.3195 μM | SANGER | |||
| RCC10RGB | Growth Inhibition Assay | IC50=13.3221 μM | SANGER | |||
| SW684 | Growth Inhibition Assay | IC50=13.9797 μM | SANGER | |||
| NCI-H1650 | Growth Inhibition Assay | IC50=15.0447 μM | SANGER | |||
| EW-13 | Growth Inhibition Assay | IC50=15.2021 μM | SANGER | |||
| EC-GI-10 | Growth Inhibition Assay | IC50=15.8644 μM | SANGER | |||
| CHP-126 | Growth Inhibition Assay | IC50=17.1918 μM | SANGER | |||
| NCI-H226 | Growth Inhibition Assay | IC50=18.0403 μM | SANGER | |||
| NCI-H1838 | Growth Inhibition Assay | IC50=20.1848 μM | SANGER | |||
| EW-11 | Growth Inhibition Assay | IC50=24.2718 μM | SANGER | |||
| EB2 | Growth Inhibition Assay | IC50=31.5318 μM | SANGER | |||
| D-502MG | Growth Inhibition Assay | IC50=43.9229 μM | SANGER | |||
| U-698-M | Growth Inhibition Assay | IC50=48.8671 μM | SANGER | |||
| ES3 | Growth Inhibition Assay | IC50=49.3893 μM | SANGER | |||
| PC3 | Function assay | Inhibition of PI3Kalpha in human PC3 cells expressing Akt1 S473D mutant assessed as phosphorylation of Akt Thr308 by immunoblotting, EC50 = 1.8 μM. | 21322566 | |||
| TC32 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for TC32 cells | 29435139 | |||
| A673 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for A673 cells | 29435139 | |||
| BT-37 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-37 cells | 29435139 | |||
| OHS-50 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for OHS-50 cells | 29435139 | |||
| Rh41 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Rh41 cells | 29435139 | |||
| SJ-GBM2 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SJ-GBM2 cells | 29435139 | |||
| SK-N-MC | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SK-N-MC cells | 29435139 | |||
| NB-EBc1 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for NB-EBc1 cells | 29435139 | |||
| SK-N-MC | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for SK-N-MC cells | 29435139 | |||
| TC32 | qHTS assay | qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Confirmatory screen for TC32 cells | 29435139 | |||
| Klik om meer experimentele gegevens over cellijnen te bekijken | ||||||
Chemische informatie, opslag en stabiliteit
| Molecuulgewicht | 607.62 | Formule | C35H28F3N5O2 |
Opslag (vanaf de datum van ontvangst) | |
|---|---|---|---|---|---|
| CAS-nr. | 1222998-36-8 | SDF downloaden | Opslag van stamoplossingen |
|
|
| Synoniemen | N/A | Smiles | CCC(=O)N1CCN(CC1)C2=C(C=C(C=C2)N3C(=O)C=CC4=CN=C5C=CC(=CC5=C43)C6=CC7=CC=CC=C7N=C6)C(F)(F)F | ||
Oplosbaarheid
|
In vitro |
DMSO
: 3 mg/mL
(4.93 mM)
Water : Insoluble Ethanol : Insoluble |
Molariteitscalculator
Verdunningscalculator
Molecuulgewichtcalculator
|
In vivo |
|||||
In vivo formulatiecalculator (heldere oplossing)
Stap 1: Voer onderstaande informatie in (Aanbevolen: een extra dier om rekening te houden met verlies tijdens het experiment)
mg/kg
g
μL
Stap 2: Voer de in vivo formulering in (Dit is alleen de calculator, geen formulering. Neem eerst contact met ons op als er geen in vivo formulering is in de sectie oplosbaarheid.)
% DMSO
%
% Tween 80
% ddH2O
%DMSO
%
Berekeningsresultaten:
Werkconcentratie: mg/ml;
Methode voor het bereiden van DMSO-moedervloeistof: mg geneesmiddel vooropgelost in μL DMSO ( Concentratie moedervloeistof mg/mL, Neem eerst contact met ons op als de concentratie de DMSO-oplosbaarheid van de batch van het geneesmiddel overschrijdt. )
Methode voor het bereiden van in vivo formulering: Neem μL DMSO moedervloeistof, voeg daarna toeμL PEG300, mengen en verhelderen, daarna toevoegenμL Tween 80, mengen en verhelderen, daarna toevoegen μL ddH2O, mengen en verhelderen.
Methode voor het bereiden van in vivo formulering: Neem μL DMSO moedervloeistof, voeg daarna toe μL Maïsolie, mengen en verhelderen.
Opmerking: 1. Zorg ervoor dat de vloeistof helder is voordat u het volgende oplosmiddel toevoegt.
2. Zorg ervoor dat u het/de oplosmiddel(en) in de juiste volgorde toevoegt. U moet ervoor zorgen dat de verkregen oplossing, bij de vorige toevoeging, een heldere oplossing is voordat u verdergaat met het toevoegen van het volgende oplosmiddel. Fysieke methoden zoals vortexen, ultrasoon of een warmwaterbad kunnen worden gebruikt om het oplossen te bevorderen.
Werkingsmechanisme
| Targets/IC50/Ki |
mTORC1
(Cell-free assay) 2 nM
mTOR
(Cell-free assay) 4.32 nM
DNA-PK
(Cell-free assay) 6.34 nM
mTORC2
(Cell-free assay) 10 nM
p110γ
(Cell-free assay) 171 nM
C2α
(Cell-free assay) 176 nM
|
|---|---|
| In vitro |
Torin1 remt de fosforylering van mTORC1- en mTORC2-substraten in cellen bij concentraties van respectievelijk 2 en 10 nM. Bovendien vertoont Torin1 een 1000-voudige selectiviteit voor mTOR ten opzichte van PI3K (EC50 = 1800 nM) en een 100-voudige bindingsselectiviteit ten opzichte van 450 andere proteïnekinasen. Torin1 veroorzaakt celcyclusarrest via een rapamycine-resistent mechanisme dat ook onafhankelijk is van mTORC2. Torin1 verstoort mTORC1-afhankelijke fenotypen vollediger dan rapamycine. Rapamycine-resistente functies van mTORC1 zijn vereist voor cap-afhankelijke translatie. In een recente studie wordt gemeld dat Torin1 de secretie van neurotensine en genexpressie verhoogt door activering van de MEK/ERK/c-Jun-route in de menselijke endocriene cellijn BON.
|
| Kinase Assay |
mTORC1- en mTORC2 in vitro kinase assays
|
|
Om oplosbaar mTORC1 te produceren, worden HEK-293T-cellijnen die stabiel N-terminaal FLAG-getagd Raptor tot expressie brengen, gegenereerd met behulp van vesiculair stomatitisvirus G-gepseudotypeerd MSCV-retrovirus. Voor mTORC2 worden vergelijkbare HeLa-cellen die stabiel N-terminaal FLAG-getagd Protor-1 tot expressie brengen, gegenereerd. Beide complexen worden gezuiverd door cellen te lyseren in 50 mm HEPES, pH 7.4, 10 mm natriumpyrofosfaat, 10 mm natrium β-glycerofosfaat, 100 mm NaCl, 2 mm EDTA, 0,3% CHAPS. Cellen worden gedurende 30 min bij 4 °C gelyseerd, en de onoplosbare fractie wordt verwijderd door microcentrifugatie bij 13.000 tpm gedurende 10 min. Supernatanten worden 1 uur geïncubeerd met FLAG-M2 monoklonale antilichaam-agarose en vervolgens driemaal gewassen met lysisbuffer en eenmaal met lysisbuffer die een uiteindelijke concentratie van 0,5 M NaCl bevat. Gezuiverd mTORC1 wordt geëlueerd met 100 μg/mL 3×FLAG-peptide in 50 mm HEPES, pH 7.4, 100 mm NaCl. Eluaat kan worden gealiquoteerd en bewaard bij -80 °C. Substraten S6K1 en Akt1 worden gezuiverd. Kinase assays worden gedurende 20 min bij 30 °C uitgevoerd in een eindvolume van 20 μL bestaande uit de kinasebuffer (25 mm HEPES, pH 7.4, 50 mm KCl, 10 mm MgCl2, 500 μm ATP) en 150 ng inactieve S6K1 of Akt1 als substraten. Reacties worden gestopt door toevoeging van 80 μL samplebuffer en gedurende 5 min gekookt. Monsters worden vervolgens geanalyseerd door SDS-PAGE en immunoblotting.
|
|
| In vivo |
Torin1 is werkzaam in een dosis van 20 mg/kg in een U87MG xenograftmodel en toont een goede farmacodynamische remming van downstream effectoren van mTOR in tumor- en perifere weefsels.
|
Referenties |
|
Toepassingen
| Methoden | Biomarkers | Afbeeldingen | PMID |
|---|---|---|---|
| Western blot | Vinculin / p-S6 p-mTOR(S2481) / mTOR / rictor / Sin1 / p-Akt S473 / p-Akt T450 / p-Akt T308 / p-S6K1 T389 / S6K1 LC3 / p62 / PARP / pAKT(S473) / p4EBP1(S65) / pS6K1(T389) / pS6(S235/236) ATF4 / p-S6K(T389) / S6K / p-eIF2α(S51) / eIF2α / 4EBP1 / eIF3b |
|
28757207 |
| Immunofluorescence | lipin-1 |
|
28347999 |
| Growth inhibition assay | Cell viability |
|
28757207 |
Technische ondersteuning
Tel: +1-832-582-8158 Ext:3
Als u nog andere vragen heeft, laat dan een bericht achter.
Veelgestelde vragen
Vraag 1:
How to re-constitute it for i.p. administration?
Antwoord:
It can be dissolved in 5% DMSO/30% PEG 300/ddH2O at 0.25 mg/ml clearly, and in 10% DMSO/corn oil at 0.5 mg/ml, it is also a clear solution.
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