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3-Methyladenine (3-MA) Autophagy/PI3K-remmer

Name: 3-Methyladenine (3-MA)
Brand: Selleck Chemicals
SKU: S2767
Rating: 5 (956 reviews)

Cat.nr.S2767

3-Methyladenine (3-MA) is een selectieve PI3K-remmer voor Vps34 en PI3Kγ met een IC50 van 25 μM en 60 μM in HeLa-cellen. Het blokkeert klasse I PI3K consistent, terwijl de onderdrukking van klasse III PI3K transiënt is, en remt ook de vorming van autophagosome. Oplossingen zijn onstabiel en moeten vers worden bereid.

3-Methyladenine (3-MA) PI3K remmer Chemical Structure

Chemische structuur

Molecuulgewicht: 149.15

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Batch: Zuiverheid: 99.97%

99.97

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Overig PI3K Inhibitoren	GDC-0077 (Inavolisib) SAR405 Quercetin (Sophoretin) LY294002 XL147 analogue Tersolisib (STX-478) Buparlisib (BKM120) 740 Y-P (PDGFR 740Y-P) GO-203 TFA Eganelisib (IPI-549)

Celkweek, behandeling & werkzame concentratie

Cellijnen	Assaytype	Concentratie	Incubatietijd	Formulering	Activiteitsbeschrijving	PMID
K562	Function Assay	10mM	1h		decreases the expression of LC3-II and the formation of autophagosomes	21864037
Jurkat	Function Assay	10mM	1h		decreases the expression of LC3-II and the formation of autophagosomes	21864037
HeLa	Cytotoxicity Assay	2mM	24h		inhibites the cytotoxicity of silibinin to HeLa cells.	21875385
PC12/TetOn	Function Assay	0.1/1mM	18h		leads to α-syn(WT) accumulation, toxicity, and oligomer formation	21906659
RMPI8226	Function Assay	5mM	1h		suppresses the level of autophagy under nutrient depletion	21915620
MCF-7	Function Assay	10mM	48h		blocks autophagy induced by bortezomib	21931937
HBx	Apoptosis Assay	10mM	48h	DMSO	increases cell death	22020078
Marc-145	Function Assay	5mM	12/24/36h		reduces the PRRSV titers and the protein expression	22119900
U937	Function Assay	2mM	12h		decreases the autophagy ratio	22155150
BGC-823	Function Assay	5mM	2h		inhibits the rate of autophagic cells	22322152
A549	Function Assay	0.1mM	24h		suppresses SU11274-induced cell death	22466960
pDCs	Function Assay	10mM	0.5h		reduces the induction of IFN-α by ssRNA40	22396599
HeLa	Apoptosis Assay	5mM	24h		induces caspase-dependent cell death	22545128
U251	Apoptosis Assay	5mM	24h		increases S1-induced cell death	22579788
MCF-7	Apoptosis Assay	0.1mM	6h		enhances sirtinol-induced apoptosis	22751989
PC-3	Apoptosis Assay	2mM	2h		increases ORI-induced cell death	22745580
HCT116	Apoptosis Assay	5mM	24h	DMSO	enhances apigenin-induced cell death	24626522
U2OS	Growth Inhibition Assay	10mM	24h		intensifies the growth inhibition induced by Dox	24639013
A2780cp	Apoptosis Assay	2.5mM	1h	ddH2O	enhances cisplatin-induced cell death	24817946
HepG2	Function Assay	5mM	4h		increases cellular levels of HL	24713587
Microglia	Apoptosis Assay	5mM	24h		decreases hypoxia-induced cell death	24818601
MDA-MB 231	Apoptosis Assay	5mM	0.5h		modulates Tocomin® induced apoptosis	24830781
PANC-1	Apoptosis Assay	1mM	48h	DMSO	enhances bortezomib-induced cell viability loss	24842158
MDA-MB-231	Function Assay	2mM	48h		promotes TM-induced cell death	24970676
MDA-MB-231	Function Assay	2mM	24h		inhibits autophagy induced by TM	24970676
MCF-7	Function Assay	2mM	48h		promotes TM-induced cell death	24970676
MCF-7	Function Assay	2mM	24h		inhibits autophagy induced by TM	24970676
HepG2	Apoptosis Assay	3mM	5h		reduces cell apoptosis induced by QDs	22836595
HeLa	Apoptosis Assay	10mM	2h		decreases cell viability co-treatment with PEI	23000135
SK-HEP-1	Apoptosis Assay	10mM	1h		protects against autophagy and induces apoptosis in bufalin-treated cells	22858649
MDA-MB231	Function Assay	5mM	1h		increases resveratrol-mediated caspase activation and cell death	23088850
PaCa44	Apoptosis Assay	2.5mM	1h		reduces genipin-mediated apoptosis	23124112
T-47D	Function Assay	10mM	2h		inhibits autophagy process and increases rapamycin induced apoptosis	23300026
GTL-16	Apoptosis Assay	5mM	24h		reduces cell viability as compared to cells treated with MET inhibitors	23313490
U251MG	Function Assay	3mM	1h		suppresses LC3-II protein expression	23338618
T24	Function Assay	10mM	1h		reduces the cleavage of LC3 after baicalin treatment	23354080
HUVECs	Function Assay	3mM	24h		blocks the protective effect of resveratrol by inhibiting autophagy	23358928
MCF-7	Function Assay	5mM	24h		inhibits starvation-induced autophagy	23395679
Hela	Function Assay	5mM	24h		inhibits starvation-induced autophagy	23395679
OR6	Function Assay	10mM	72h		suppresses HCV replication and formation of autophagosomes	23395875
HT-29	Function Assay	1mM	48/96h		inhibits AMPK induces autophagic cell death	23508272
SH-SY5Y	Cytotoxicity Assay	5mM	24h		increases PCN toxicity	23525265
Saos-2	Apoptosis Assay	1mM	96h		increases cell death induced by PCX	23563171
1321N1	Cytotoxicity Assay	5mM	24h		protects cell against PCN-induced toxicity	23525265
A2780	Apoptosis Assay	5mM	24h		converts FTY720 with CDDP into an additive effect towards killing ovarian cancer cells	23592281
OV2008	Apoptosis Assay	5mM	24h		converts FTY720 with CDDP into an additive effect towards killing ovarian cancer cells	23592281
PC12	Function Assay	10mM	24h	water	inhibits chymotrypsin-like proteasomal activity.	23603979
SH-SY5Y	Apoptosis Assay	5mM	1h		abolishes celastrol neuroprotective effect	23619395
SH-SY5Y	Function Assay	1mM	24h		inhibits the autophagy induced by TOCP	23743148
HepG2	Function Assay	10mM	24h		inhibits siTIGAR- and HBSS-induced autophagy	23817040
HeLa	Function Assay	10mM	2h		suppresses LC3 II expressison	23864738
HONE-1	Function Assay	5mM	1h		represses 6r-mediated ROS production	23892358
MCF7	Function Assay	5mM	24h		increases CuO induced cell death	23962629
HO8910	Apoptosis Assay	10mM	12h		enhances B19-induced apoptosi	23983610
SMMC-7721	Apoptosis Assay	5mM	24h		attenuates TNF-α protection against serum starvation-mediated apoptosis	24066693
Hep3B	Apoptosis Assay	5mM	24h		attenuates TNF-α protection against serum starvation-mediated apoptosis	24066693
H460	Function Assay	10mM	4h		increases cisplatin-induced cell death	24173208
A549	Function Assay	10mM	4h		inhibits autophagy induced by irradiation	24142735
H1299	Function Assay	10mM	4h		increases cisplatin-induced cell death	24173208
WiDr	Function Assay	10mM	1h		inhibits PCBL-induced LC3 II expression	24190489
LoVo	Apoptosis Assay	5mM	48h		enhances DCA-induced apoptosis.	24201812
HepG2 E47	Function Assay	2.5mM	48h		increases the toxicity of AA, BSO, and CCl4	24273738
RKO	Function Assay	2mM	1h	DMSO	enhances cell death by geldanamycin	24291777
Hep3B	Apoptosis Assay	2mM	12h	DMSO	inhibits AZD8055-induced cell death	24297300
ACHN-5968	Apoptosis Assay	5mM	3h		enhances paclitaxel-mediated apoptosis	24305604
Huh7	Apoptosis Assay	2mM	12h	DMSO	inhibits AZD8055-induced cell death	24297300
UOK257	Apoptosis Assay	5mM	3h		enhances paclitaxel-mediated apoptosis	24305604
ECSCs	Apoptosis Assay	10mM	4h		decreases rapamycin-treated apoptosis	24319109
MCF-7	Function Assay	10mM	24h		inhibits the autophagy induced by chemotherapy drugs	24315578
SGC-7901	Apoptosis Assay	2mM	1h		increases CA-4 induced apoptosis	24321340
SMMC-7721	Apoptosis Assay	2mM	1h		increases CA-4 induced apoptosis	24321340
T24	Apoptosis Assay	5mM	1.5h		potentiates celecoxib-induced apoptosis	24349176
NTUB1	Apoptosis Assay	5mM	1.5h		potentiates celecoxib-induced apoptosis	24349176
MG-63	Apoptosis Assay	10mM	12h		enhances DP-induced apoptosis	24358301
MG-63	Apoptosis Assay	0.5/1mM	32h		enhances salinomycin-induced cell apoptosis	24358342
MG-63	Function Assay	0.5/1mM	48h		induces salinomycin-induced cell viability loss	24358342
U2OS	Function Assay	0.5/1mM	48h		induces salinomycin-induced cell viability loss	24358342
HGC-27	Function Assay	10mM	1h		inhibits the cell viability loss by RAD001 or MK-2206	24416349
HCT116	Apoptosis Assay	5mM	24h		enhances the apoptosis induced by apigenin	24626522
A549	Apoptosis Assay	10mM	48h		accelerates the reduction of cell viability induced by PTX	24626722
Saos-2	Apoptosis Assay	10mM	24h		intensifies the growth inhibition of the U2OS cells induced by Dox	24639013
U2OS	Apoptosis Assay	10mM	24h		intensifies the growth inhibition of the U2OS cells induced by Dox	24639013
HepG2	Function Assay	5mM	4h		increases HL release	24713587
A549	Apoptosis Assay	5mM	48h		decreases the percentage of cell death and expression levels of caspase-3, Beclin-1 and LC3-II	24706303
A2780cp	Apoptosis Assay	2.5mM	1h	ddH2O	enhances cisplatin-induced cell death	24817946
Microglia	Apoptosis Assay	5mM	24h		decreases hypoxia-induced cell death	24818601
HT-29	Apoptosis Assay	1mM	48h	DMSO	enhances bortezomib-induced cell viability loss	24842158
MDR	Apoptosis Assay	10mM	6h		strengthens the power of anticancer drugs	25019701
H157	Function Assay	5mM	2h		suppresses SPC induced accumulation of LC3-II	25285628
A549	Function Assay	5mM	2h		suppresses SPC induced accumulation of LC3-II	25285628
A2780cp	Growth Inhibition Assay	1mM	1h		increases cisplatin-induced cell death	25322694
NBL-W-S	Apoptosis Assay	1mM	6h		increases cell apoptosis induced by GANT-61	25323222
NBL-W-S	Growth Inhibition Assay	1mM	6h		enhances GANT-61 toxicity	25323222
A549	Apoptosis Assay	5mM	2h	DMSO	inhibits BDMC-induced apoptotic cell death	25716561
95D	Apoptosis Assay	5mM	2h	DMSO	inhibits BDMC-induced apoptotic cell death	25716561
A549	Growth Inhibition Assay	3mM	2h	DMSO	reduces growth inhibitory effect of BDMC	25716561
95D	Growth Inhibition Assay	3mM	2h	DMSO	reduces growth inhibitory effect of BDMC	25716561
Nara-H	Growth Inhibition Assay	5mM	48h		enhances temsirolimusmediated suppression of Nara-H cell proliferation	21805033
HUVECs	Function Assay	10mM	0.5h		decreases the AGE-BSAinduced autophagy leve	21468592
HepG2	Apoptosis Assay	2mM	1h		enhances radiation-induced cell death	21453691
U-2 OS	qHTS assay				qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for U-2 OS cells	29435139
A673	qHTS assay				qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for A673 cells	29435139
DAOY	qHTS assay				qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for DAOY cells	29435139
Saos-2	qHTS assay				qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Saos-2 cells	29435139
BT-37	qHTS assay				qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-37 cells	29435139
RD	qHTS assay				qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for RD cells	29435139
SK-N-SH	qHTS assay				qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SK-N-SH cells	29435139
BT-12	qHTS assay				qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for BT-12 cells	29435139
MG 63 (6-TG R)	qHTS assay				qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for MG 63 (6-TG R) cells	29435139
NB1643	qHTS assay				qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for NB1643 cells	29435139
OHS-50	qHTS assay				qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for OHS-50 cells	29435139
Rh41	qHTS assay				qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Rh41 cells	29435139
Rh30	qHTS assay				qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Rh30 cells	29435139
SJ-GBM2	qHTS assay				qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SJ-GBM2 cells	29435139
SK-N-MC	qHTS assay				qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for SK-N-MC cells	29435139
NB-EBc1	qHTS assay				qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for NB-EBc1 cells	29435139
LAN-5	qHTS assay				qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for LAN-5 cells	29435139
Rh18	qHTS assay				qHTS of pediatric cancer cell lines to identify multiple opportunities for drug repurposing: Primary screen for Rh18 cells	29435139
Klik om meer experimentele gegevens over cellijnen te bekijken

Chemische informatie, opslag en stabiliteit

Molecuulgewicht	149.15	Formule	C₆H₇N₅	Opslag (vanaf de datum van ontvangst)	3 years -20°C powder
CAS-nr.	5142-23-4	SDF downloaden		Opslag van stamoplossingen	Oplossingen zijn instabiel. Bereid ze vers of koop kleine, voorverpakte formaten. Herverpakken na ontvangst.
Synoniemen	NSC 66389			Smiles	CN1C=NC(=N)C2=C1N=CN2

Oplosbaarheid

In vitro
Batch:

DMSO : 10 mg/mL (67.04 mM) Verwarmd met een waterbad van 50°C; Geultrasoneerd;
(Met vocht verontreinigd DMSO kan de oplosbaarheid verminderen. Gebruik verse, watervrije DMSO.)

Ethanol : 10 mg/mL

Water : 4 mg/mL

DMSO : 16 mg/mL (107.27 mM) Verwarmd met een waterbad van 50°C; Geultrasoneerd;
(Met vocht verontreinigd DMSO kan de oplosbaarheid verminderen. Gebruik verse, watervrije DMSO.)

Water : 10 mg/mL (超声加热十分钟，60℃)

Ethanol : 8 mg/mL

Molariteitscalculator

Massa	Concentratie	Volume	Molecuulgewicht
=	×	×

Verdunningscalculator Molecuulgewichtcalculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Gevalideerd door Selleck labs. Mocht u aanpassingen aan deze formulering nodig hebben, neem dan contact op met ons verkoopteam voor aangepaste tests.	0.750mg/ml (5.03mM)	Taking the 1 mL working solution as an example, add 50 μL of 15 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Gevalideerd door Selleck labs. Mocht u aanpassingen aan deze formulering nodig hebben, neem dan contact op met ons verkoopteam voor aangepaste tests.	0.500mg/ml (3.35mM)	Taking the 1 mL working solution as an example, add 50 μL of 10 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.

In vivo formulatiecalculator (heldere oplossing)

Stap 1: Voer onderstaande informatie in (Aanbevolen: een extra dier om rekening te houden met verlies tijdens het experiment)

Dosering: mg/kg Gemiddeld gewicht van dieren: g Doseervolume per dier:μL Aantal dieren:

Stap 2: Voer de in vivo formulering in (Dit is alleen de calculator, geen formulering. Neem eerst contact met ons op als er geen in vivo formulering is in de sectie oplosbaarheid.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Berekeningsresultaten:

Werkconcentratie: mg/ml;

Methode voor het bereiden van DMSO-moedervloeistof: mg geneesmiddel vooropgelost in μL DMSO ( Concentratie moedervloeistof mg/mL, Neem eerst contact met ons op als de concentratie de DMSO-oplosbaarheid van de batch van het geneesmiddel overschrijdt. )

Methode voor het bereiden van in vivo formulering: Neem μL DMSO moedervloeistof, voeg daarna toeμL PEG300, mengen en verhelderen, daarna toevoegenμL Tween 80, mengen en verhelderen, daarna toevoegen μL ddH₂O, mengen en verhelderen.

Methode voor het bereiden van in vivo formulering: Neem μL DMSO moedervloeistof, voeg daarna toe μL Maïsolie, mengen en verhelderen.

Opmerking: 1. Zorg ervoor dat de vloeistof helder is voordat u het volgende oplosmiddel toevoegt.
2. Zorg ervoor dat u het/de oplosmiddel(en) in de juiste volgorde toevoegt. U moet ervoor zorgen dat de verkregen oplossing, bij de vorige toevoeging, een heldere oplossing is voordat u verdergaat met het toevoegen van het volgende oplosmiddel. Fysieke methoden zoals vortexen, ultrasoon of een warmwaterbad kunnen worden gebruikt om het oplossen te bevorderen.

Werkingsmechanisme

Targets/IC₅₀/Ki	Autophagy Vps34 (HeLa cells) 25 μM PI3Kγ (HeLa cells) 60 μM
In vitro	De lichte voorkeur voor Vps34-preventie door 3-Methyladenine (3-MA) komt waarschijnlijk voort uit een hydrofobe ring die specifiek is voor Vps34 en die de 3-methylgroep van deze verbinding omgeeft. Het is gemeld dat het kankerceldood veroorzaakt onder zowel normale als uithongeringscondities, en zou ook celmigratie en invasie kunnen onderdrukken onafhankelijk van zijn vermogen om Autophagy te remmen, wat impliceert dat het functies bezit anders dan Autophagy-onderdrukking. Deze verbinding ontlokt caspase-afhankelijke celdood die onafhankelijk is van Autophagy-remming. Behandeling met 5 mM ervan vermindert het percentage glucose-uitgehongerde HeLa-cellen dat GFP-LC3-punctae vertoont tot 23%. De niveaus van LC3-I nemen toe en de niveaus van LC3-II nemen af tussen 12 en 48 uur in cellen die met 3-MA zijn behandeld. Omzetting van LC3-I naar LC3-II wordt onderdrukt door de verbinding. Behandeling van HeLa-cellen ermee bij 2,5 mM of 5 mM gedurende één dag heeft geen invloed op de cellevensvatbaarheid, terwijl behandeling met 10 mM gedurende één dag een afname van 25,0% in cellevensvatbaarheid veroorzaakt. Behandeling van cellen met 2,5, 5 of 10 mM gedurende twee dagen veroorzaakt respectievelijk 11,5%, 38,0% en 79,4% afname in levensvatbaarheid. Het vermindert de cellevensvatbaarheid op een tijd- en dosisafhankelijke manier en verkort de duur van nocodazol-geïnduceerde-prometafase-arrestatie significant. Onderdrukking van Autophagy door 3-MA remt SU11274-geïnduceerde celdood. Langdurige behandeling ermee (tot 9 uur) induceert significante LC3 I naar II conversie in wildtype MEF's. Langdurige behandeling met 3-MA, maar niet met wortmannine, verhoogt de GFP-LC3-punctatie/aggregatie aanzienlijk. De geïnduceerde LC3-conversie en vrije GFP-vrijlating zijn ATG7-afhankelijk. Behandeling ermee leidt tot een duidelijke toename van het p62-eiwitniveau. De verbinding verhoogt het p62-niveau zelfs in Atg5−/− MEF's en in cellen met DOX-gemedieerde deletie van ATG5. Het remt klasse I en klasse III PI3K in verschillende temporele patronen. De geïnduceerde LC3 I naar LC3 II conversie is dramatisch gecompromitteerd in Tsc2−/− cellen vergeleken met wildtype cellen. Deze verbinding verstoort de anti-autofagische functie van het mTOR-complex 1.
Kinase Assay	Eiwitafbraaktest
Kinase Assay	HeLa-cellen worden gedurende 24 uur radiogelabeld met 0,05 mCi/mL l-[U-¹⁴C]valine. Aan het einde van de labelingsperiode worden de cellen driemaal gespoeld met PBS. Cellen worden gedurende de aangegeven tijden geïncubeerd in ofwel volledig medium of EBSS met of zonder de aanwezigheid van 10 mM 3-Methyladenine (3-MA).
In vivo	3-Methyladenine (3-MA) blokkeert Autophagy via zijn effect op klasse III phosphatidylinositol 3-kinase (PI3K). Behandeling met deze verbinding verandert de mate van bloeding niet vergeleken met de subarachnoïdale bloeding (SAH) groep. De voorbehandeling ervan verergert neurologische symptomen significant in vergelijking met de SAH + voertuig groep. Autophagy is verminderd wanneer het wordt toegepast. Omgekeerd is gekliefde caspase-3 significant opgereguleerd in de SAH + 3-MA groep. In lijn met de opregulatie van gekliefde caspase-3 expressie, is het aantal TUNEL-positieve cellen in de rechter cortex significant verhoogd in de SAH + 3-MA groep vergeleken met de SAH + voertuig groep.
Referenties	[1] https://pubmed.ncbi.nlm.nih.gov/20574157/ [2] https://pubmed.ncbi.nlm.nih.gov/22545128/ [3] https://pubmed.ncbi.nlm.nih.gov/22466960/ [4] https://pubmed.ncbi.nlm.nih.gov/20123989/ [5] https://pubmed.ncbi.nlm.nih.gov/22521819/

Toepassingen

Methoden	Biomarkers	PMID
Western blot	α-SMA / TGF-β / LC-3BI / LC-3B II / Beclin-1 / NF-κB p65 caspase-3 / caspase-9 / PARP VEGF APP / BACE1 / ADAM17 / Presenilin 1 / Presenilin 2 / Nicastrin / APH-1 / Pen-2 / LC3-1 / LC3-2	29296191
Immunofluorescence	LC3 / Hif-α / COX2	29039446
Growth inhibition assay	Cell viability	26934124

Technische ondersteuning

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Tel: +1-832-582-8158 Ext:3

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Veelgestelde vragen

Vraag 1:
I'm also wondering whether it can be dissolved in water, or maybe something like culture medium, normal saline solution to form 10mM solution.

Antwoord:
As the reference (http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal. pone.0035665), it was found to inhibit autophagy at concentrations ranging from 1 to 10 mM and was directly dissolved into the culture medium at the indicated concentrations. And we tested the solubility of S2767, and found its solubility in DMEM is 31 mg/mL at about 40°C.

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