Name: DMOG (Dimethyloxallyl Glycine)
Brand: Selleck Chemicals
SKU: S7483
Rating: 5 (52 reviews)

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Batch: Zuiverheid: 99.92%

99.92

Gerelateerde doelwitten	Dehydrogenase HSP Transferase P450 (e.g. CYP17) PDE phosphatase PPAR Vitamin Carbohydrate Metabolism Mitochondrial Metabolism
Overig Hydroxylase Inhibitoren	Ro 61-8048 Meldonium 4-Chloro-DL-phenylalanine Nepicastat (SYN-117) HCl 2-Thiouracil (R)-Nepicastat HCl H-Tyr(3-I)-OH

Celkweek, behandeling & werkzame concentratie

Cellijnen	Assaytype	Concentratie	Activiteitsbeschrijving	PMID
human SR cell	Growth inhibition assay		Inhibition of human SR cell growth in a cell viability assay, IC50=4.57946 μM	SANGER
human LB996-RCC cell	Growth inhibition assay		Inhibition of human LB996-RCC cell growth in a cell viability assay, IC50=5.6877 μM	SANGER
human LOUCY cell	Growth inhibition assay		Inhibition of human LOUCY cell growth in a cell viability assay, IC50=10.3493 μM	SANGER
human KS-1 cell	Growth inhibition assay		Inhibition of human KS-1 cell growth in a cell viability assay, IC50=11.2557 μM	SANGER
human U-698-M cell	Growth inhibition assay		Inhibition of human U-698-M cell growth in a cell viability assay, IC50=13.3306 μM	SANGER
human A3-KAW cell	Growth inhibition assay		Inhibition of human A3-KAW cell growth in a cell viability assay, IC50=15.8174 μM	SANGER
human BL-70 cell	Growth inhibition assay		Inhibition of human BL-70 cell growth in a cell viability assay, IC50=15.9423 μM	SANGER
human DMS-273 cell	Growth inhibition assay		Inhibition of human DMS-273 cell growth in a cell viability assay, IC50=15.965 μM	SANGER
human CAL-39 cell	Growth inhibition assay		Inhibition of human CAL-39 cell growth in a cell viability assay, IC50=19.3277 μM	SANGER
human KYSE-410 cell	Growth inhibition assay		Inhibition of human KYSE-410 cell growth in a cell viability assay, IC50=20.8197 μM	SANGER
human YT cell	Growth inhibition assay		Inhibition of human YT cell growth in a cell viability assay, IC50=23.3984 μM	SANGER
human LC-2-ad cell	Growth inhibition assay		Inhibition of human LC-2-ad cell growth in a cell viability assay, IC50=24.5573 μM	SANGER
human COR-L105 cell	Growth inhibition assay		Inhibition of human COR-L105 cell growth in a cell viability assay, IC50=26.4436 μM	SANGER
human CAL-12T cell	Growth inhibition assay		Inhibition of human CAL-12T cell growth in a cell viability assay, IC50=26.5502 μM	SANGER
human KU812 cell	Growth inhibition assay		Inhibition of human KU812 cell growth in a cell viability assay, IC50=28.4579 μM	SANGER
human OPM-2 cell	Growth inhibition assay		Inhibition of human OPM-2 cell growth in a cell viability assay, IC50=28.8227 μM	SANGER
human SW982 cell	Growth inhibition assay		Inhibition of human SW982 cell growth in a cell viability assay, IC50=33.7007 μM	SANGER
human HH cell	Growth inhibition assay		Inhibition of human HH cell growth in a cell viability assay, IC50=34.0235 μM	SANGER
human RS4-11 cell	Growth inhibition assay		Inhibition of human RS4-11 cell growth in a cell viability assay, IC50=34.1806 μM	SANGER
human BEN cell	Growth inhibition assay		Inhibition of human BEN cell growth in a cell viability assay, IC50=36.8187 μM	SANGER
human CCRF-CEM cell	Growth inhibition assay		Inhibition of human CCRF-CEM cell growth in a cell viability assay, IC50=37.7729 μM	SANGER
human EW-13 cell	Growth inhibition assay		Inhibition of human EW-13 cell growth in a cell viability assay, IC50=39.0481 μM	SANGER
human NCI-H2228 cell	Growth inhibition assay		Inhibition of human NCI-H2228 cell growth in a cell viability assay, IC50=42.818 μM	SANGER
human KE-37 cell	Growth inhibition assay		Inhibition of human KE-37 cell growth in a cell viability assay, IC50=43.2915 μM	SANGER
human A4-Fuk cell	Growth inhibition assay		Inhibition of human A4-Fuk cell growth in a cell viability assay, IC50=45.0364 μM	SANGER
human MZ1-PC cell	Growth inhibition assay		Inhibition of human MZ1-PC cell growth in a cell viability assay, IC50=45.9888 μM	SANGER
human MLMA cell	Growth inhibition assay		Inhibition of human MLMA cell growth in a cell viability assay, IC50=46.3292 μM	SANGER
human EB-3 cell	Growth inhibition assay		Inhibition of human EB-3 cell growth in a cell viability assay, IC50=49.5792 μM	SANGER
human 293T cells	Function assay	2.5 mM	Inhibition of JMJD2-mediated H3K36 demethylation in human 293T cells at 2.5 mM	19359167
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Chemische informatie, opslag en stabiliteit

Molecuulgewicht	175.14	Formule	C₆H₉NO₅	Opslag (vanaf de datum van ontvangst)	3 years -20°C powder
CAS-nr.	89464-63-1	SDF downloaden		Opslag van stamoplossingen	Oplossingen zijn instabiel. Bereid ze vers of koop kleine, voorverpakte formaten. Herverpakken na ontvangst.
Synoniemen	Dimethyloxalylglycine			Smiles	COC(=O)CNC(=O)C(=O)OC

Oplosbaarheid

In vitro
Batch:

DMSO : 35 mg/mL (199.84 mM)
(Met vocht verontreinigd DMSO kan de oplosbaarheid verminderen. Gebruik verse, watervrije DMSO.)

Water : 35 mg/mL

Ethanol : 35 mg/mL

Molariteitscalculator

Massa	Concentratie	Volume	Molecuulgewicht
=	×	×

Verdunningscalculator Molecuulgewichtcalculator

In vivo Batch:
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Homogeneous suspension	CMC-NA	≥5mg/ml	Taking the 1 mL working solution as an example, add 5 mg of this product to 1 ml of CMC-Na solution, mix evenly to obtain a homogeneous suspension with a final concentration of 5 mg/ml.
Clear solution	5%DMSO 1 40%PEG300 2 5%Tween80 3 50%ddH2O Gevalideerd door Selleck labs. Mocht u aanpassingen aan deze formulering nodig hebben, neem dan contact op met ons verkoopteam voor aangepaste tests.	1.750mg/ml (9.99mM)	Taking the 1 mL working solution as an example, add 50 μL of 35 mg/ml clarified DMSO stock solution to 400 μL of PEG300, mix evenly to clarify it; add 50 μL of Tween80 to the above system, mix evenly to clarify it; then continue to add 500 μL of ddH2O to adjust the volume to 1 mL. The mixed solution should be used immediately for optimal results.

In vivo formulatiecalculator (heldere oplossing)

Stap 1: Voer onderstaande informatie in (Aanbevolen: een extra dier om rekening te houden met verlies tijdens het experiment)

Dosering: mg/kg Gemiddeld gewicht van dieren: g Doseervolume per dier:μL Aantal dieren:

Stap 2: Voer de in vivo formulering in (Dit is alleen de calculator, geen formulering. Neem eerst contact met ons op als er geen in vivo formulering is in de sectie oplosbaarheid.)

% DMSO + % + % Tween 80 + % ddH₂O

%DMSO+ %

Berekeningsresultaten:

Werkconcentratie: mg/ml;

Methode voor het bereiden van DMSO-moedervloeistof: mg geneesmiddel vooropgelost in μL DMSO ( Concentratie moedervloeistof mg/mL, Neem eerst contact met ons op als de concentratie de DMSO-oplosbaarheid van de batch van het geneesmiddel overschrijdt. )

Methode voor het bereiden van in vivo formulering: Neem μL DMSO moedervloeistof, voeg daarna toeμL PEG300, mengen en verhelderen, daarna toevoegenμL Tween 80, mengen en verhelderen, daarna toevoegen μL ddH₂O, mengen en verhelderen.

Methode voor het bereiden van in vivo formulering: Neem μL DMSO moedervloeistof, voeg daarna toe μL Maïsolie, mengen en verhelderen.

Opmerking: 1. Zorg ervoor dat de vloeistof helder is voordat u het volgende oplosmiddel toevoegt.
2. Zorg ervoor dat u het/de oplosmiddel(en) in de juiste volgorde toevoegt. U moet ervoor zorgen dat de verkregen oplossing, bij de vorige toevoeging, een heldere oplossing is voordat u verdergaat met het toevoegen van het volgende oplosmiddel. Fysieke methoden zoals vortexen, ultrasoon of een warmwaterbad kunnen worden gebruikt om het oplossen te bevorderen.

Werkingsmechanisme

Targets/IC₅₀/Ki	HIF prolyl hydroxylase
In vitro	DMOG toont slechts zwakke activiteit in het microsomale systeem, maar onderdrukt efficiënt de hydroxyprolinesynthese in intacte cellen. Deze verbinding vermindert FGF-2-geïnduceerde proliferatie en cycline A-expressie door de prolylhydroxylase-activiteit in HPASMC te remmen.
In vivo	DMOG remt endogene HIF-inactivatie en induceert angiogenese in ischemische skeletspieren van muizen. Opregulatie van hypoxie-induceerbare factor-1α door deze verbinding versterkt de cardioprotectieve effecten van ischemische postconditionering bij hyperlipidemische ratten.
Referenties	[1] https://pubmed.ncbi.nlm.nih.gov/8002959/ [2] https://pubmed.ncbi.nlm.nih.gov/15319416/ [3] https://pubmed.ncbi.nlm.nih.gov/19304911/ [4] https://pubmed.ncbi.nlm.nih.gov/24389644/

Toepassingen

Methoden	Biomarkers	Afbeeldingen	PMID
Western blot	HIF-1α p-CHK1 / CHK1 / p-p53 / p53 / p-CHK2 / CHK2 / p-ATM / ATM WNT11 / HIF-2α		29555474
Immunofluorescence	HIF1α		28955984

Technische ondersteuning

Gebruiksaanwijzing

Tel: +1-832-582-8158 Ext:3

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DMOG (Dimethyloxallyl Glycine) HIF-PH Remmer

Chemische structuur

Molecuulgewicht: 175.14